Semnan University of Medical Sciences

Research and Technology Vice Chancellor

Aghazadeh, Mohammad and Samadi Kafil, Hossein and Ghotaslou, Reza and Asgharzadeh, Mohammad and Moghadami, Maryam and Akhi, Mohammad Taghi and Hojabri, Zoya and Naghili, Behrouz and Najafi, Khadijeh and Azimi, Somayeh and Shokrian, Saeed (2016) Prevalence of oxacillinase groups i, ii and iii in Pseudomonas aeruginosa isolates by polymerase chain reaction and genotyping by ERIC-PCR methods. Jundishapur Journal of Microbiology. pp. 1-6.

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    Abstract

    Background: Pseudomonas aeruginosa is main bacterial pathogen accountable for nosocomial infections. Furthermore, it could potentially become resistant to β-lactams, aminoglycosides and fluoroquinolones antibiotics. Objectives: The purpose of this study was to determine the antibiotic susceptibility pattern and genotyping of P. aeruginosa in hospitals of Tabriz (Iran) and investigate the prevalence of OXA producer isolates. Methods: Overall, 151 non-replicated isolates of P. aeruginosa were collected from October 2013 until September 2014. Antibiotic susceptibility pattern was determined by disk diffusion (Kirby Bauer) method, according to the clinical laboratory standards institute (CLSI) guideline. Genes encoding OXA (Ambler class D) β-lactamase were detected by PCR for all isolates. Polymerase chain reaction with Enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) primers was used to establish the clonal relationship between the different isolates. Results: The frequencies of resistance to antibiotics were as follows: gentamicin: 68%, ceftazidime: 67%, piperacillin: 66%, cefepime: 64%, ciprofloxacin: 62%, tobramycin: 61%, amikacin: 60%, imipenem: 52%, gatifloxacin: 28%, polymyxin B: 2 % and colistin: 2%. The OXA group I genes was identified in 82 (56%), the OXA group II gene in 26 (18%), OXA group III in 14 (9%), OXA-1 in 22 (15%) and OXA-4 in 3 (2%) isolates. The ERIC-PCR indicated high genetic diversity among P. aeruginosa isolates. Conclusions: The high prevalence of OXA β-lactamase and high genetic diversity of P. aeruginosa indicated that the resistance of P. aeruginosa might be expanding in our studied hospitals. Keywords: Antibiotic; Pseudomonas aeruginosa; Genotyping; Beta-Lactamase OXA; Polymerase Chain Reaction

    Item Type: Article
    Subjects: R Medicine > R Medicine (General)
    Divisions: Faculty of Medical Sciences > School of Medicine
    Depositing User: Mr Vahab Moshtaghi
    Date Deposited: 19 Apr 2017 10:22
    Last Modified: 19 Apr 2017 10:22
    URI: http://eprints.semums.ac.ir/id/eprint/883

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