Semnan University of Medical Sciences

Research and Technology Vice Chancellor

Rezvany, Mohammad Reza and Kazemi, Azam and Hajifathali, Abbas and Kaviani, Saeed and Mellstedt, Hakan (2007) Analysis of HLA-G gene expression in B-lymphocytes from chronic lymphocytic leukemia patients. Source of the Document Iranian Biomedical Journal. pp. 125-129.

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    Abstract

    The human leukocyte antigen G (HLA-G) molecule exhibits limited tissue distribution, low polymorphism and alternative splicings that generate seven HLA-G isoforms. HLA-G exerts multiple immunoregulatory functions. Recent studies indicate an ectopic up-regulation in tumor cells that may favor their escape from anti-tumor immune responses. This study it is an effort to clarify the presence of HLA-G in B-cell chronic lymphocytic leukemia (B-CLL) patients. Methods: HLA-G mRNA expression was studied in a pilot study in circulating B-CLL and also healthy controls by reverse transcription (RT)-PCR using a set of pan-HLA-gamma primers. Results: RT-PCR was performed on B-cells from 74 B-CLL patients and 12 healthy controls. The data showed HLA-G gene expression in 20% of the B-CLL patients. No expression of HLA-G could be detected in the healthy control group. Conclusion: These data suggest that HLA-G is expressed at the gene level in B cells from B-CLL patients but not in B cells from healthy controls. Further study is required to clarify the role of HLA-gamma as a regulatory factor that could affect immune response in B-CLL patients. Keywords: Human leukocyte antigen G (HLA-G), Reverse transcription PCR (RT)-PCR, B-cell chronic lymphocytic leukemia (B-CLL)

    Item Type: Article
    Subjects: R Medicine > R Medicine (General)
    Divisions: Faculty of Medical Sciences > School of Medicine
    Depositing User: Mr Vahab Moshtaghi
    Date Deposited: 05 Feb 2017 10:11
    Last Modified: 05 Feb 2017 10:11
    URI: http://eprints.semums.ac.ir/id/eprint/812

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