Semnan University of Medical Sciences

Research and Technology Vice Chancellor

Dokouhaki, Pouneh and Moghaddam, Rosa and Rezvany, MohammadReza and Ghassemi, Jamileh and Novin, Marefat Ghaffari and Zarnani, Amirhassan and Akhondi, Mohammah-Mehdi and Ostadkarampour, Mahyar and Mellstedt, Håkan and Razavi, Alireza and Jeddi-Tehrani, Mahmood (2008) ORIGINAL ARTICLE: Repertoire and Clonality of T-Cell Receptor Beta Variable Genes Expressed in Endometrium and Blood T Cells of Patients with Recurrent Spontaneous Abortion. American Journal of Reproductive Immunology, 60 (2). pp. 160-171. ISSN 10467408

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    PROBLEM:Recurrent spontaneous abortion (RSA) is a relatively common disorder, the underlying causes of which are thought to be immunological in most cases. METHOD OF STUDY:Expression profile and clonality pattern of T-cell receptor beta variable (TCRBV) genes in endometrium and blood of patients with RSA were investigated by semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) using BV gene-specific primers. Relative expression of each BV family was determined and clonal expansion of the over-expressed genes was assessed by analysis of CDR3 length polymorphism. RESULTS:Compared to blood, relative expression of four TCRBV genes was significantly higher in the endometrium of RSA group. Over-expressed genes, except for TCRBV3, all had restricted and oligoclonal patterns of expression in the endometrium. CONCLUSION:Endometrial T cells have a skewed TCRBV repertoire with restricted transcript heterogeneity, which is shared by both groups and minor variations observed in this pattern in RSA patients may reflect more recent and/or repeated exposure to nominal antigens or superantigens.

    Item Type: Article
    Subjects: R Medicine > R Medicine (General)
    Divisions: Faculty of Medical Sciences > School of Medicine
    Depositing User: Mr Vahab Moshtaghi
    Date Deposited: 08 Jan 2017 09:41
    Last Modified: 08 Jan 2017 09:41

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