Semnan University of Medical Sciences

Research and Technology Vice Chancellor

Kebriaei, Abdollah and Derakhshan, Mohammad and Meshkat, Zahra and Eidgahi, Mohammad Reza Akbari and Rezaee, Seyed Abdolrahim and Farsiani, Hadi and Mosavat, Arman and Soleimanpour, Saman and Ghazvini, Kiarash (2016) Construction and immunogenicity of a new Fc-based subunit vaccine candidate against Mycobacterium tuberculosis. Molecular Biology Reports, 43 (9). pp. 911-922. ISSN 0301-4851

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    Abstract

    As an ancient disease, tuberculosis (TB) is a major global health threat. Therefore, there is an urgent need for an effective and safe anti-TB vaccine. In the current study, a delivery system of Fc domain of mouse IgG2a and early secreted antigenic target protein 6 (ESAT-6) was evaluated for the selective uptake of antigens by antigen-presenting cells (APCs). Thus, it was based on the immunogenicity of a fusion protein. The study was initiated by the transfer of recombinant expression vectors of pPICZαA-ESAT-6:Fcγ2a and pPICZαA-ESAT-6: His into Pichia pastoris (P. pastoris). Recombinant proteins were assessed for immunogenicity following the immunoblotting analysis. High levels of IFN-γ and IL-12 were produced to induce Th1-type cellular responses through vaccination with both recombinant proteins [ESAT-6:Fcγ2a (EF) and ESAT-6:His (EH)]. The Fc-tagged recombinant protein induced more effective Th1-type cellular responses with a low increment in IL-4 compared to PBS, BCG, and EH groups. Although in all the immunized groups, the ratio of IFN-γ/IL-4 was in favor of Th1 responses, the highest Th1/Th2 balance was observed in EF immunized group. Fc fragment of mouse IgG2a may induce a selective uptake of APCs towards the cross-presentation and formation of Th1 responses in favor of an appropriate protective anti-tuberculosis reaction. Thus, further research on Fc-fusion proteins is required to develop Fc-based TB vaccines.

    Item Type: Article
    Subjects: R Medicine > R Medicine (General)
    Divisions: Faculty of Medical Sciences > School of Medicine
    Depositing User: Admin User
    Date Deposited: 04 Sep 2016 07:57
    Last Modified: 04 Sep 2016 07:57
    URI: http://eprints.semums.ac.ir/id/eprint/68

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