Semnan University of Medical Sciences

Research and Technology Vice Chancellor

Pourmohammadi, B and Motazedian, MH and Hatam, GR and Kalantari, M and Habibi, P and Sarkari, B (2010) Comparison of three methods for diagnosis of cutaneous leishmaniasis. Iranian Journal of Parasitology. pp. 1-8.

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    Abstract

    BACKGROUND:Leishmaniasis is one of the infectious parasitic diseases of highest incidence in the world. Cutaneous Leishmaniasis (CL) has long been reported in Shiraz, Southern Iran. There is a need to find a sensitive and specific method for treatment and control of the disease. METHODS:We have compared the sensitivity of the conventional methods microscopy and cultivation of lesion scrapes against PCR amplification of parasite kinetoplast DNA from these samples. The samples (n=219) were obtained from the patients clinically suspected of CL. The smears were stained with Giemsa for microscopy and cultured in Novy-Nicolle-McNeal (NNN) blood agar for promastigote growth. For PCR, the dry smears were scraped off the slides and DNA was extracted. RESULTS:The positive rates from 219 specimens were 76.71%, 50.68%, and 93.61% for microscopy, cultivation, and PCR, respectively. The highest correlation was found between PCR and microscopy method (P=0.014). In PCR assay, 95.61%, 3.9%, and 0.49% of the samples were identified as Leishmania major, L. tropica, and dermatropic L. infantum, respectively. CONCLUSION:The PCR method appears to be the most sensitive for the diagnosis of CL and is valuable for identifying the other species of Leishmania with confusing dermatropic signs. KEYWORDS:Cultivation; Cutaneous Leishmaniasis; Diagnosis; PCR

    Item Type: Article
    Subjects: R Medicine > R Medicine (General)
    Divisions: Faculty of Medical Sciences > School of Medicine
    Depositing User: Mr Vahab Moshtaghi
    Date Deposited: 14 Dec 2016 02:15
    Last Modified: 14 Dec 2016 02:15
    URI: http://eprints.semums.ac.ir/id/eprint/623

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