Semnan University of Medical Sciences

Research and Technology Vice Chancellor

Moradi, Fateme and Bahktiari, Mehrdad and Joghataei, Mohammad Taghi and Nobakht, Maliheh and Soleimani, Masoud and Hasanzadeh, Gholamreza and Fallah, Ali and Zarbakhsh, Sam and Hejazian, Leila Beigom and Shirmohammadi, Maryam and Maleki, Fatemeh (2012) BD PuraMatrix peptide hydrogel as a culture system for human fetal Schwann cells in spinal cord regeneration. Journal of Neuroscience Research, 90 (12). pp. 2335-2348. ISSN 03604012

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    Abstract

    BD PuraMatrix peptide hydrogel, a three-dimensional cell culture model of nanofiber scaffold derived from the self-assembling peptide RADA16, has been applied to regenerative tissue repair in order to develop novel nanomedicine systems. In this study with PuraMatrix, self-assembling nanofiber scaffold (SAPNS) and Schwann cells (SCs) were isolated from human fetal sciatic nerves, cultured within SAPNS, and then transplanted into the spinal cord after injury (SCI) in rats. First, the peptide nanofiber scaffold was evaluated via scanning electron microscopy and atomic force microscopy. With phase-contrast microscopy, the appearance of representative human fetal SCs encapsulated in PuraMatrix on days 3, 5, and 7 in 12-well plates was revealed. The Schwann cells in PuraMatrix were cultured for 2 days, and the SCs had active proliferative potential. Spinal cord injury was induced by placing a 35-g weight on the dura of T9-T10 segments for 15 min, followed by in vivo treatment with SAPNS and human fetal SCs (100,000 cells/10 μl/injection) grafted into spinal cord 7 days after SCI. After treatment, the recovery of motor function was assessed periodically using the Basso, Beattie, and Bresnahan scoring system. Eight weeks after grafting, animals were perfusion fixed, and the survival of implanted cells was analyzed with antibody recognizing SCs. Immunohistochemical analysis of grafted lumber segments at 8 weeks after grafting revealed reduced asterogliosis and considerably increased infiltration of endogenous S100(+) cells into the injury site, suggesting that PuraMatrix may play an important role in the repair observed after SAPNS and human fetal SC transplantation. Copyright © 2012 Wiley Periodicals, Inc.

    Item Type: Article
    Subjects: R Medicine > R Medicine (General)
    Divisions: Faculty of Medical Sciences > School of Medicine
    Depositing User: Mr Vahab Moshtaghi
    Date Deposited: 20 Nov 2016 20:53
    Last Modified: 20 Nov 2016 20:53
    URI: http://eprints.semums.ac.ir/id/eprint/496

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