Semnan University of Medical Sciences

Research and Technology Vice Chancellor

Abedi, E. and Kheirandish, M. and Sharifi, Z. and Samiee, S. and Kokhaei, P. and Pourpak, Z. and Ashraf, M.J. (2015) Quantitative polymerase chain reaction for detection of human herpesvirus-7 infection in umbilical cord blood donors. Transplant Infectious Disease, 17 (1). pp. 21-24. ISSN 13982273

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    Abstract

    Objective: Umbilical cord blood (UCB) has been a reasonable alternative to granulocyte colony-stimulating factor-mobilized peripheral blood or bone marrow, as a source of hematopoietic stem cells with a lower risk of graft-versus-host disease. In immunocompromised hosts after transplantation, the risk of viral infection in adults, especially with beta-herpesviruses such as human herpesvirus-7 (HHV-7), may be increased. This virus in immunocompromised patients can be reactivated from latency and converted to an active phase. Therefore, light-upon-extension real-time polymerase chain reaction (PCR) was developed to assess the prevalence and load of HHV-7 in the plasma and buffy coat of donors. Methods: About 825 UCB samples under standard protocol from donors were collected. Then, DNA from plasma and buffy coat was extracted and quantitative real-time PCR was performed with light-upon-extension primers. Results: Overall, HHV-7 was detected in 3.64% (30/825) of UCB donors. HHV-7 DNA was detected in 26 (3.2%) buffy coat samples (latent infection), and only 4 (0.48%) of them were positive for HHV-7 DNA in plasma samples (active infection); the mean HHV-7 viral load was 1.31 × 101 copies/mL in latent infection, and 1.94 × 105 copies/mL in active infection. Conclusions: We suggest that real-time PCR in plasma and buffy coat could be a useful method to detect active and latent HHV-7 infection in UCB donors and determine its role in subsequent transmission events.

    Item Type: Article
    Subjects: R Medicine > R Medicine (General)
    Divisions: Faculty of Medical Sciences > School of Medicine
    Depositing User: Mr Vahab Moshtaghi
    Date Deposited: 27 Sep 2016 07:04
    Last Modified: 27 Sep 2016 07:04
    URI: http://eprints.semums.ac.ir/id/eprint/233

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