Semnan University of Medical Sciences

Research and Technology Vice Chancellor

Hojabri, Z. and Arab, M. and Darabi, N. and Kia, N.S. and Lopes, B.S. and Pajand, O. (2019) Evaluation of the commercial combined disk test and minimum inhibitory concentration (MIC) determination for detection of carbapenemase producers among Gram-negative bacilli isolated in a region with high prevalence of bla OXA-48 and bla NDM. International Microbiology. pp. 81-89.

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    Carbapenem-resistant Gram-negative bacilli (GNB) are a concern in the Middle East and worldwide. Simple screening methods have been sought to detect carbapenemase producers to determine appropriate therapeutic measures and implement infection control interventions. In this study, we evaluated the efficiency of agar disc diffusion, commercial combined disc test (Rosco), and carbapenem MIC determination in comparison to molecular detection of carbapenemase genes among 82 carbapenem non-susceptible Enterobacteriaceae (CNSE) and 37 Acinetobacter/Pseudomonas isolates. The bla OXA-48 , bla NDM , bla NDM/OXA-48 , and bla IMP were detected in 68 out of 82 CNSE isolates. All of the Acinetobacter baumannii isolates were positive for the bla OXA-51 (n = 23), of those some were positive for bla OXA-48 (n = 13) and bla NDM (n = 3). Sensitivities and specificities of combined disc test for detection of bla NDM and bla OXA-48 carrying Enterobacteriaceae isolates were 92.5% and 100%, and 58.5% and 100%, respectively, while those for Acinetobacter/Pseudomonas isolates were 100%, 81.8% and 96.2%, 89%, respectively. While carbapenem MIC values had excellent concordance with phenotypic combined disc test for detection of bla OXA-48 producers (area under curve > 90%), only ertapenem MIC’s could precisely detect bla OXA-48 PCR-positive Enterobacteriaceae isolates (AUC 70%, sensitivity 70%, specificity 50%). The phenotypic commercial test showed excellent sensitivity for detection of bla NDM producers, but had poor sensitivity for bla OXA-48 -producing Enterobacteriaceae. Ertapenem MIC values had low sensitivity and specificity for detection of the bla OXA-48 -carrying Enterobacteriaceae. This is the first report of A. baumannii isolates co-harbored the bla OXA-48 /bla NDM carbapenemases from Iran. © 2018, Springer Nature Switzerland AG.

    Item Type: Article
    Subjects: R Medicine > R Medicine (General)
    Divisions: Faculty of Medical Sciences > School of Medicine
    Depositing User: Admin User
    Date Deposited: 13 Mar 2019 03:28
    Last Modified: 13 Mar 2019 03:28

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