Semnan University of Medical Sciences

Research and Technology Vice Chancellor

Mansour, Reyhaneh Nassiri and Barati, Ghasem and Soleimani, Masoud and Ghoraeian, Pegah and Nouri Aleagha, Maryam and Kehtari, Mousa and Mahboudi, Hossein and Hosseini, Fatemeh and Hassannia, Hadi and Abazari, Mohammad Foad and Enderami, Seyed Ehsan (2018) Generation of high-yield insulin producing cells from human-induced pluripotent stem cells on polyethersulfone nanofibrous scaffold. Artificial Cells, Nanomedicine, and Biotechnology. pp. 1-7. ISSN 2169-1401 (In Press)

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Abstract

Transplantation of islet is a promising method in treatment of patients with type 1 diabetes mellitus (T1DM), however, is limited by islet shortage. The aim of this study was to prepare a polyethersulfone (PES) nanofibrous scaffolds to evaluate the pancreatic differentiation of human induced pluripotent stem cells (hiPSCs). The differentiation process in tissue culture dishes and PES scaffolds was evaluated at mRNA and protein level by RT-qPCR and immunofluorescence assay, respectively. The functionality of differentiated cells was determined by insulin and C-peptide release in response to glucose challenges. The results of this study showed that cells cultured on PES nanofibrous scaffolds exhibit more pancreatic β-cell characteristics as they express more pancreatic tissue-specific genes and proteins. Furthermore, the immunoassay showed that differentiated cells in both culture plates and PES scaffolds groups are functional and secrete C-peptide and insulin in response to glucose challenges. Altogether, the results of this study demonstrated that PES nanofibrous scaffold could provide the microenvironment that promotes the differentiation of induced pluripotent stem cells (iPSCs) into insulin producing cells. KEYWORDS:Induced pluripotent stem cells; insulin-producing cells; nanofibrous scaffold; polyethersulfone

Item Type: Article
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medical Sciences > School of Medicine
Depositing User: Mr Vahab Moshtaghi
Date Deposited: 30 Apr 2018 14:03
Last Modified: 23 May 2018 09:55
URI: http://eprints.semums.ac.ir/id/eprint/1381

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