Semnan University of Medical Sciences

Research and Technology Vice Chancellor

Abdolshahi, Anna and Tabatabaie yazdi, Farideh and Shabani, Ali Akbar and Mortazavi, Seyed Ali and Marvdashti, Leila Monjazeb (2018) Aflatoxin binding efficiency of Saccharomyces cerevisiae mannoprotein in contaminated pistachio nuts. Food Control, 87. pp. 17-21. ISSN 09567135

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    Mannoprotein (mannan), an important cell wall component of Saccharomyces cerevisiae, is a bioactive glycoprotein that has aflatoxin binding effect. In this study, mannan from the cell wall of Saccharomyces cerevisiae was extracted and lyophilized. Aflatoxin binding assay of mannan in water/methanol were spiked with 0.5 μl. Aflatoxin standard solution showed that aflatoxin binding and mannan concentration had a positive correlation (p < 0.05). To evaluate the binding efficacy of mannan on aflatoxin content in pistachio, the contaminated pistachio kernels were soaked in mannan solution (25 and 50 mg/ml). Highest binding percent (84.40 ± 0.20% for aflatoxin B1) was seen in higher concentration of mannan. An increasing trend was observed by contact time (5, 15, 30, 60,120, 180 min); furthermore, there were 80% aflatoxin binding occurrence in the first 5 min contact time. The aflatoxin binding efficacy of mannan incorporated in gelatin -based coating on pistachio showed that the gelatin/mannan (3.5:1.5) coating can bind 83.49 ± 0.17% aflatoxin in contaminated pistachio. Since aflatoxin contamination of nuts such as pistachio is a critical defect in quality of control and safety guarantee, elimination/reduction of aflatoxin content attracted more attentions. According the results of this study, mannan alone or in corporation with gelatin -based coating has a potential application to reduce aflatoxin level in contaminated pistachio.

    Item Type: Article
    Subjects: R Medicine > R Medicine (General)
    Divisions: Faculty of Medical Sciences > School of Medicine
    Depositing User: Mr Vahab Moshtaghi
    Date Deposited: 30 Apr 2018 09:39
    Last Modified: 26 Dec 2018 10:11

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