Semnan University of Medical Sciences

Research and Technology Vice Chancellor

Shahrezaie, Mostafa and Mansour, Reyhaneh Nassiri and Nazari, Bahare and Hassannia, Hadi and Hosseini, Fatemeh and Mahboudi, Hossein and Eftekhary, Mohamad and Kehtari, Mousa and Veshkini, Arash and Ahmadi Vasmehjani, Abbas and Enderami, Seyed Ehsan (2017) Improved stem cell therapy of spinal cord injury using GDNF-overexpressed bone marrow stem cells in a rat model. Biologicals, 50. pp. 73-80. ISSN 10451056

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Abstract

The use of stem cell base therapy as an effective strategy for the treatment of spinal cord injury (SCI) is very promising. Although some strategy has been made to generate neural-like cells using bone marrow mesenchymal stem cells (BMSCs), the differentiation strategies are still inefficiently. For this purpose, we improved the therapeutic outcome with utilize both of N-neurotrophic factor derived Gelial cells (GDNF) gene and differentiation medium that induce the BMSCs into the neural-like cells. The differentiated GDNF overexpressed BMSCs (BMSCs-GDNF) were injected on the third day of post-SCI. BBB score test was performed for four weeks. Two weeks before the end of BBB, biotin dextranamin was injected intracrebrally and at the end of the fourth week, the tissue was stained. BBB scores were significantly different in BMSCs-GDNF injected and control animals. Significant difference in axon counting was observed in BMSCs-GDNF treated animals compared to the control group. According to the results, differentiated BMSCs-GDNF showed better results in comparison to the BMSCs without genetic modification. This study provides a new strategy to investigate the role of simultaneous in stem cell and gene therapy for future neural-like cells transplantation base therapies for SCI. KEYWORDS: Bone marrow mesenchymal stem cells; Cell therapy; GDNF; Gene therapy; Spinal cord injury

Item Type: Article
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medical Sciences > School of Medicine
Depositing User: Mr Vahab Moshtaghi
Date Deposited: 24 Apr 2018 12:50
Last Modified: 21 May 2018 12:34
URI: http://eprints.semums.ac.ir/id/eprint/1238

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