Semnan University of Medical Sciences

Research and Technology Vice Chancellor

Aghaeepoor, Mojtaba and Kobarfard, Farzad and Eidgahi, Mohammad Reza Akbari and Shabani, Aliakbar and Dehnavi, Ehsan and Ahmadi, Maryam and Akbarzadeh, Ali (2017) Optimization of culture media for extracellular expression of streptokinase in Escherichia coli using response surface methodology in combination with Plackett-Burman Design. Tropical Journal of Pharmaceutical Research, 16 (11). p. 2567. ISSN 1596-5996

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Abstract

Purpose: To investigate the enhancement of streptokinase extracellular expression in Escherichia coli by adjusting culture media. Methods: Screening of 10 chemical factors (EDTA, peptone, glycine, triton X-100, glycerol, K2HPO4, KH2PO4, Ca2+ (calcium chloride), yeast and NaCl) in order to increase the secretion of extracellular protein was carried out by response surface methodology (RSM). The method was also employed to optimize the concentrations of critical factors that had been determined in the screening step. Results: The results indicate that glycine, triton X-100 and Ca2+ were the most effective chemical factors in terms of increase in extracellular expression of streptokinase with optimum levels of 0.878, 0.479 and 0.222 %, respectively. Expression of streptokinase under optimum concentrations of critical permeabilizing factors led to a 7-fold increase in the quantity of secreted recombinant protein (5824 U/mL) compared to the initial level (802 U/mL). Conclusion: The results show that medium optimization using RSM is effective in improving extracellular streptokinase expression. The optimization medium is considered fundamental and useful for efficient production of streptokinase on a large scale. Keywords: Streptokinase, Response surface methodology, Membrane permeabilization, Extracellular secretion

Item Type: Article
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medical Sciences > School of Medicine
Depositing User: Mr Vahab Moshtaghi
Date Deposited: 24 Apr 2018 12:51
Last Modified: 21 May 2018 12:34
URI: http://eprints.semums.ac.ir/id/eprint/1231

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