Semnan University of Medical Sciences

Research and Technology Vice Chancellor

Ghahremanfard, Farahnaz and Aghabeigi, Arash and Manouchehri, Ehsan and Pashaei, Mehrnoosh and Mirmohammadkhani, Majid and Shokrollahi Barough, Mahdieh and Pak, Fatemeh and Faranoush, Mohammad and Kokhaei, Parviz (2017) Effect of berberine on cancer cell viability of chronic lymphocytic leukemia patients in in vitro. Koomesh. pp. 634-640.

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    Introduction:Chronic lymphocytic leukemia (CLL) is the most common leukemia in the Western world. CLL generally affects older people by the accumulation and proliferation of B lymphocytes with markers CD19+, CD20+, CD5+ and CD23+ specified. In this way, berberine, including natural compounds with isoquinoline alkaloids structure, that has a potential anti-cancer effect. Berberin decreased survival and increased apoptosis of cancer cell lines in in vitro. In this study, the effect of berberine on cell survival and apoptosis in PBMC patients and healthy donors in the presence or absence of berberine and also comparison between bernerine and fludarabine were investigated. Materials and Methods:Peripheral blood mononuclear cells (PBMCs) of 12 patients and 6 healthy donors were isolated by using ficoll technique and PBMC cells seeded into the 96 well culture plate using medium RPMI1640+ AB+ serum with doses ranging from 5 to 200 μM of Berberine. The survival and apoptosis percentage of these cells was measured by MTT and Annexin V/7AAD (Flowcytometery). Results:Our results suggest a reduction in PBMC survival in CLL patients treated with berberine with P<0.05 as compared to patients cells which not treated. Comparing PBMC survival treated with berberine to cells treated with fludarabine showed no significant change (P>0.05). Correspondingly, increasing PBMC apoptosis in patients treated with berberine was observed rather than the cells were not adjacent to berberine (P<0.05) and also compared with PBMC apoptosis treated with fludarabine which shown no significant difference (P >0.05). However, we don’t have this increasing in healthy donors. Conclusion: Berberine leads to reduced viability and elevated levels of apoptosis in PBMCs of CLL Patients. Berberin showed no significant effects on PBMCs of healthy donor viability and apoptosis. Apoptosis induction by berberin is comparable to fludarabine as a standard treatment for CLL patients. Berberine might be a good candidate for clinical trial for CLL patients Keywords: B-Cell Chronic Lymphocytic Leukemia, Berberine, Fludarabine, Apoptosis, Annexin 5A

    Item Type: Article
    Subjects: R Medicine > R Medicine (General)
    Divisions: Faculty of Medical Sciences > School of Medicine
    Depositing User: Mr Vahab Moshtaghi
    Date Deposited: 05 Jul 2017 12:11
    Last Modified: 05 Jul 2017 12:11

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